This product is for research use only not for diagnostic or in vivo use. The Re-Blot Plus Western Blot Mild Antibody Stripping Solution should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction site. The MILLIPORE Re-Blot Plus Western Blot Mild Antibody Stripping Solution is effective for removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes. Mild Antibody Stripping Solution (10x) - (2 containers / 25 mL each).It is recommended that one new strip (not subjected to stripping solution) be included for comparison purposes. Positive and Negative Stripping Controls.Plastic Trays for incubation of blots or blot strips in stripping, washing and blocking solutions.Plastic Wrap, such as Saran Wrap, for storage of blots that will not be re-probed immediately.Standard Blot or blot strips, on nitrocellulose or PVDF/nylon membrane.*Blots can be stripped of antibodies in approximately 15 minutes at room temperature.*Antibody stripping is done at room temperature.*No pungent-smelling b-mercaptoethanol is contained in the Antibody Stripping Solution.The MILLIPORE Re-Blot Plus Western Blot Mild Antibody Stripping Solution contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins.Īdvantages of the Re-Blot Plus Western Blot Mild Antibody Stripping Solution include: While antigen and antibody-based immunoaffinity matrices, such as Sepharose™ conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded the application of these methods to Western blotting. subtype- or isoform-specific antibodies,ģ) Re-analysis of anomalous results and confirmation with the same or a different antibody,Ĥ) Correcting errors in incubation with the wrong antibody,ĥ) Cost savings in reagents and time by reusing the same blot. Stripping and re-probing of Western blots offers several advantages:ġ) Conservation of samples that are expensive or available only in limited quantities,Ģ) Analysis of a given blot using several different antibodies, e.g. Unlike nucleic acid based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots. Typically, protein samples are electro-phoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Western blotting is a commonly used technique for studying protein function and localization. ReBlot Plus Mild Antibody Stripping Solution, 10x
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